Blood plasma analysis - Analysis of drugs in blood plasma
Direct injection of plasma samples onto a HPLC column is likely to result in adsorption of plasma proteins to the stationary phase with resulting gradual loss in retention and column efficiency. Restricted access media type pre-column and column switching has been used for direct injection of plasma samples on reversed phase LC systems.However, still, there was a continuous degradation of the column performance. However, with the Nanosep Technology, the interaction phase is exchanged after every analysis. Thus, every new sample is injected onto a column containing a fresh interaction phase and adsorption of sample components to the interaction phase will therefore not affect the next analysis. The technique thus has great potential for analysis of samples accompanied with complex matrices.
Theophylline in blood plasma analysed using the Nanosep RP particles.
